Immobilized pancreatic lipase and colipase for purification and binding studies
نویسندگان
چکیده
منابع مشابه
Binding of bile salts to pancreatic colipase and lipase.
The binding of conjugated bile salts to pancreatic colipase and lipase has been studied by equilibrium dialysis and gel filtration. The results indicate that at physiological ionic strength and pH, conjugated bile salts bind as micelles to colipase: 12-15 moles/mole of colipase for the dihydroxy conjugates and 2-4 for the trihydroxy conjugates. No binding of bile salt takes place from monomeric...
متن کاملBinding of porcine pancreatic lipase and colipase in the absence of substrate studies by two-phase partition and affinity chromatography.
The binding of porcine pancreatic lipase and colipase was studied using the techniques of two-phase partition and affinity chromatography. The binding exhibited a pH dependency (maximum = pH 5.8, minimum = pH 8.6), was not significantly effected by the concentrations of NaCl, (0 to 1 M), and was inhibited by bile salts at concentrations above their critical micelle concentration. Below their cr...
متن کاملSpecific serum pancreatic lipase determination, with use of purified colipase.
We show that the turbidimetric method of Ziegenhorn et al. (Clin. Chem. 25: 1067, 1979) for determination of pancreatic lipase is not influenced by lipoprotein lipase. This improved specificity as compared to standard lipase methods is explained by the presence of purified colipase and the high concentration of bile acids in the substrate emulsion.
متن کاملChromosomal localization of lipolytic enzymes in the mouse: pancreatic lipase, colipase, hormone-sensitive lipase, hepatic lipase, and carboxyl ester lipase.
Several lipases and their cofactors are involved in the absorption, transport, storage, and mobilization of lipids. As part of an effort to examine the role of these enzymes in plasma lipid metabolism and genetic susceptibility to atherosclerosis, we report the chromosomal mapping of their genes in mouse. Restriction fragment length variants for each gene were identified, typed in an interspeci...
متن کاملsimulation and experimental studies for prediction mineral scale formation in oil field during mixing of injection and formation water
abstract: mineral scaling in oil and gas production equipment is one of the most important problem that occurs while water injection and it has been recognized to be a major operational problem. the incompatibility between injected and formation waters may result in inorganic scale precipitation in the equipment and reservoir and then reduction of oil production rate and water injection rate. ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1978
ISSN: 0014-5793
DOI: 10.1016/0014-5793(78)80558-4